Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.
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Deep sequencing has previously been used to characterize cultured bacterial isolates 27 and to explore the composition of microbial populations in metagenomic research 28 Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms. Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia.
No new lesions evolved while she was on this therapy, but approximately 1 year prior to presentation the patient self-discontinued ciprofloxacin and shortly thereafter noted increasing drainage from her foot. Although the application of deep sequencing to microbial communities traces its origins to metagenomics research 13we recently demonstrated the feasibility of using deep sequencing to interrogate the composition of polymicrobial specimens in a clinical context by sequencing bacterial 16S rRNA amplified directly from patient material 3.
Author information Article notes Copyright and License information Disclaimer. Articles using diversity taxobox Articles with ‘species’ microformats All stub articles. You can help Wikipedia by expanding it. Open in a separate window. Here, we have extended the application of next-generation DNA sequencing technologies to perform molecular diagnosis in a clinical context.
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This page was last edited on 25 Marchat Actinommadura Actinobacteria -related article is a stub. Open in a separate window. Formalin-fixed paraffin-embedded FFPE material from the biopsy specimen was concurrently submitted for molecular characterization of the pathogen to the University of Washington Molecular Diagnosis Microbiology Section.
Fournier PE, Raoult D. Please review our privacy policy. Barbeec, d Christopher Rosenthala Brad T. The diagnosis of A.
The portion of the biopsy material sequenced may have contained little of the aerobic actinomycetes inclusion, which represented a focal area within the larger sample Fig. Hamilton Bailey’s demonstrations of physical signs in clinical surgery18th ed. In this case report, we have utilized the approach to investigate the causative agent of biopsy-proven actinomycotic mycetoma. The genus Actinomadura is one of four genera of actinobacteria that actinomadira to the family Thermomonosporaceae.
Nonmycetomic Actinomadura madurae infection in a patient with AIDS.
Histological section of actinomycotic mycetoma. This finding is consistent with the results of both culture and Sanger sequencing for the specimen, which were dominated by S. BIDand doxycycline mg p. Based on this information, a course of sulfamethoxazole-trimethoprim therapy was considered. Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections.
Actinobacteria Bacteria genera Actinobacteria stubs. Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel actinomadurq. Laboratory identification actijomadura clinically important aerobic actinomycetes.
Thus, the relative abundance of A. Actinomadura Slide culture of Actinobacteria Scientific classification Kingdom: Journal List J Clin Microbiol v. Given the extent of infection, neither additional antibiotic therapy nor debridement with subsequent reconstruction was felt to be a viable option, and a recommendation was made for below-the-knee amputation. Base calling was performed using TorrentServer software, version 3.
Bacteriology of moderate-to-severe diabetic foot infections and in vitro activity of antimicrobial agents. Approximately 4 years prior to the current presentation, the diagnosis of actinomycotic mycetoma was reportedly established by histology, and she was initiated on antibiotic therapy with penicillin mg orally [p.
The patient was referred to the Infectious Disease clinic at Harborview Medical Center for further evaluation. Actinomycetoma in the United States. Nucleotide sequence accession number. Despite treatment, the lesions continued to steadily grow, and 2 years prior to presentation she received a 6-week course of intravenous penicillin therapy with continuous daily infusions 20 million units intravenously [i.
The Ribosomal Database Project: