Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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If the problem continues, please let politenicoe know and we’ll try to help. When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present. You are right about the Triton. Please sign in or create an account.

Your institution must subscribe to JoVE’s Biology section to access this content. This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect? Do you have any advice?

Los cromosomas politenicos como herramienta para el estudio de especies de la familia simulidae.

If you are seeing few salivary gland chromosome sets you may be losing them when you squash. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. Repita los pasos 4.


Agradecemos a la Sra. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas. Lugar pesos g en el cubreobjetos de las politebicos.

The trick is to leave them in just long enough. An unexpected error occurred.

Preparación de los cromosomas politénicos de Drosophila Calabazas para el Etiquetado de anticuerpos

Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido poolitenicos a la diapositiva y no quedarse con el cubreobjetos. Las modificaciones de este procedimiento se describen en detalle en Johansen et al.

Is it the PFA? Cromosomaas to load video. Please recommend JoVE to your librarian. For other languages click here. Fill out the form below to receive a free trial or learn more about access:. A subscription to J o VE is required to view this article. Skip to content Biology.

If that doesn’t help, cromosomqs let us know. That is the volume I prepare each time, usually enough for two or three prep. A squash that is too rapid or hard may also cause the chromosomes to float away. My thought is that they may be getting flushed out during my transfers. Caliente para dispersar a la Triton X y su uso dentro de una hora. You must be signed in to post a comment. If you leave the glands in too long they will fall apart.


You will only pklitenicos able to see the first 20 seconds.

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Deficiencia intelectual – catarata – cifosis

Fill out the form below to receive a free trial or learn more about access: Click here for the english version. The dissection forceps we use were mis-identified: Please check your Internet connection and reload this page. Warm to disperse the Triton X Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash.