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The construction, expression, and purification of GST-caveolin 1 fusion proteins were as we described previously 2425 Caveolin was recombinantly expressed as a glutathione S -transferase fusion. For additional information, see the Global Shipping Programme terms and conditions – opens in a new window or tab This amount includes applicable customs duties, taxes, brokerage and other fees.
To confirm that this part fits your vehicle, please choose a vehicle from the My Garage list or enter your vehicle’s details below. Thus, caveolin may organize the formation of caveolae microdomains and orchestrate caveolae-related signaling events.
Pdyn (prodynorphin) | Gene Report | BioGPS
Primary antibodies were detected using distinctly tagged fluorescent secondary antibodies: In order to define a minimal region of caveolin that can functionally support an association with c-Src, GST-caveolin fusion proteins encoding distinct portions of the N- or C-terminal domains of caveolin were used as the substrate for c-Src binding.
Similarly, Shalloway and colleagues found that auto-phosphorylation of the c-Src is an intermolecular process using phosphopeptides as a probe International postage and import charges paid to Pitney Bowes Inc.
For example, Shoelson and colleagues have shown that high concentrations of peptide substrates inhibited the autophosphorylation of c-Src kinase Submit your work to JBC. Portions of the information contained in this table have been provided by carpartsinmotion. This amino acid stretch of caveolin residues is located within a membrane-proximal region of the cytosolic N-terminal domain of caveolin.
Representative APR variable Briefly, phosphorylated protein bands were digitized by high resolution optical scanning; volumetric integration of signal intensities was carried out using ImageQuant Software Fast Scan; Molecular Dynamics.
Thus, we have termed this amino acid stretch of caveolin residues the caveolin scaffolding domain. The inhibitory effect of peptide 2 was slightly less potent on Fyn tyrosine kinase.
Mutationally cs c-Src has been implicated in the pathogenesis of carcinomas, including breast tumors Glenney for monoclonal antibodies directed against caveolin; Dr. This region is identical in c-Src and v-Src. After binding, extensive washing, and elution with reduced glutathione, bound Src was visualized by immunoblot analysis.
For additional information, see the Global Shipping Programme terms and conditions – opens in a new window or tab. Add to Watch list. Watch list is full. Putative allosteric activators could stabilize c-Src in the activated state, whereas putative allosteric inhibitors could hold c-Src in an inactive conformation Besides interacting with Src family kinases, this cytosolic caveolin domain residues has the following unique features.
Rear Axle; for PR number: The expression of Src tyrosine kinases was monitored by immunoblot analysis with a mAb that recognizes the conserved extreme N-terminal of Src. Bquantitation of A middle panel. Learn more – opens in new window or tab Seller information carpartsinmotion These experiments were performed at least three times independently in duplicate.
Subject to credit approval. One aliquot was subjected to an immune complex kinase assay see below. After binding, extensive washing, and elution with reduced glutathione, bound Src was visualized by immunoblot analysis as in Fig. In addition, our results indicate that caveolin preferentially interacts with c-Src rather than v-Src.
Add to Watch list Watching Watch list is full. However, in both reports, the investigators used much higher cs of peptides, such as 0.
After expression, c-Src kinase was isolated by immunoprecipitation and subjected to the immune complex kinase assay Fig. The effects of these peptides are shown in B.
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As such, caveolin may function as a common membrane-anchored scaffolding protein for these and other cytoplasmic signaling molecules.
Thus, it appears that caveolin structurally and functionally interacts with wild-type c-Src via caveolin residues If the item comes direct from a manufacturer, it may be delivered in non-retail packaging, such as a plain or unprinted box or plastic bag. The effects of peptide 1 A and peptide 2 B are shown. Construction and Purification of Glutathione S-Transferase-Caveolin Fusion Proteins The construction, expression, and purification of GST-caveolin 1 fusion proteins were as we described previously 2425 ZhaoLan Tang, Kenneth S.
Previous Section Next Section. Phosphorylated proteins were visualized by autoradiography using an intensifying screen. Anti-Src-immunoprecipitates from T cells co-transfected with c-Src and caveolin were washed twice with kinase reaction buffer 20 m M Hepes, pH 7. Processed by PayPal Get more time to pay.
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Using purified recombinant c-Src tyrosine kinase, we evaluated the effects of these caveolin peptides on the functional auto-activation of c-Src kinase in vitro.
These immunoprecipitates were subjected to immunoblot analysis with a Src mAb probe top panelan immune complex kinase assay to detect Src auto-phosphorylation middle paneland immunoblot analysis with a caveolin mAb probe bottom panel. Compatibility information isn’t available for this item. Deletion mutagenesis indicates that the Src-interacting domain of caveolin is located within residuesa cytosolic membrane-proximal region of caveolin.
Search for related content. In vivo effect of the full-length caveolin molecule on the auto-activation es c-Src tyrosine kinase revealed by co-expression of caveolin and c-Src in T cells. Auto-activation of c-Src occurs through auto-phosphorylation of tyrosine